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polyclonal rabbit anti-rat α-sma  (Boster Bio)


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    Boster Bio polyclonal rabbit anti-rat α-sma
    Polyclonal Rabbit Anti Rat α Sma, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti-rat+%CE%B1-sma/anti+%CE%B1+sma+bm0002/pm39696429-103-40-47
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-rat α-sma - by Bioz Stars, 2026-10
    90/100 stars

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    Activity Assay:

    Article Title: NCAM1 modulates the proliferation and migration of pulmonary arterial smooth muscle cells in pulmonary hypertension.
    Article Snippet: The endogenous peroxidase activity was blocked by incubation with 0.3% hydrogen peroxide at room temperature for 25 min. Then the sections were blocked with 3% bovine serum albumin (BSA) (Servicebio, Wuhan, China) for 30 min and then incubated with a polyclonal rabbit anti-rat α-SMA (Cat # BM0002, BOSTER Biological Technology, China) antibodies overnight, followed by incubation with the secondary antibody [Goat Anti-Rabbit IgG (H + L) -HRP, AIFang biological, Changsha, China] for 50 min. After the colour development through incubation with diaminobenzidine, the sections were counterstained with haematoxylin.

    Incubation:

    Article Title: NCAM1 modulates the proliferation and migration of pulmonary arterial smooth muscle cells in pulmonary hypertension.
    Article Snippet: The endogenous peroxidase activity was blocked by incubation with 0.3% hydrogen peroxide at room temperature for 25 min. Then the sections were blocked with 3% bovine serum albumin (BSA) (Servicebio, Wuhan, China) for 30 min and then incubated with a polyclonal rabbit anti-rat α-SMA (Cat # BM0002, BOSTER Biological Technology, China) antibodies overnight, followed by incubation with the secondary antibody [Goat Anti-Rabbit IgG (H + L) -HRP, AIFang biological, Changsha, China] for 50 min. After the colour development through incubation with diaminobenzidine, the sections were counterstained with haematoxylin.



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    Image Search Results


    Pathological and immunohistochemical results between the control and the experimental groups. (A) . The HE staining (400ⅹ). (B) . The Masson staining (400ⅹ), the fibrosis area was calculated by ImageJ software, and there was significant statistical difference between the two groups (** p < .01). (C) . Immunohistochemical staining of smooth muscle specific marker α -SMA was compared between the two groups.

    Journal: Frontiers in Physiology

    Article Title: Characteristics and transcriptomic analysis of scar tissues on the inner uterine cavity wall in patients with intrauterine adhesions

    doi: 10.3389/fphys.2022.990009

    Figure Lengend Snippet: Pathological and immunohistochemical results between the control and the experimental groups. (A) . The HE staining (400ⅹ). (B) . The Masson staining (400ⅹ), the fibrosis area was calculated by ImageJ software, and there was significant statistical difference between the two groups (** p < .01). (C) . Immunohistochemical staining of smooth muscle specific marker α -SMA was compared between the two groups.

    Article Snippet: The tissue sections were deparaffinized, treated with 3% H2O2 for 25 min to inactivate endogenous peroxidases, heated in 10-mM citrate buffer at 121°C for 30 min for antigen retrieval, blocked in 5% normal serum for 20 min, and incubated with a primary polyclonal rabbit anti-rat antibody specific for α -SMA (1:200 in PBS, Servicebio; GB111364) overnight at 4°C.

    Techniques: Immunohistochemical staining, Control, Staining, Software, Marker